PCR Assays Based on invA Gene Amplification are not Reliable for Salmonella Detection
نویسندگان
چکیده
منابع مشابه
Selective Amplification of prt, tyv and invA Genes by Multiplex PCR for Rapid Detection of Salmonella typhi
A multiplex PCR-based assay was developed for detection of Salmonella typhi and identification of other salmonella serotypes. Three primer-sets were selected from different genomic sequences, malo2-F/malo2-Ra primers from invasion gene, Parat-s/Parat-as as well as tyv-s/tyv-as primers from O-antigen gene cluster of the genus Salmonella. This method differentiated Salmonella spp., based on size ...
متن کاملSelective Amplification of prt, tyv and invA Genes by Multiplex PCR for Rapid Detection of Salmonella typhi
A multiplex PCR-based assay was developed for detection of Salmonella typhi and identification of other salmonella serotypes. Three primer-sets were selected from different genomic sequences, malo2F/malo2-Ra primers from invasion gene, Parat-s/Parat-as as well as tyv-s/tyv-as primers from Oantigen gene cluster of the genus Salmonella. This method differentiated Salmonella spp., based on size an...
متن کاملDetection of InvA Gene in Isolated Salmonella from Broilers by PCR Method
The presence of Salmonella was detected in 192 samples of poultry carcasses from poultry farms in Shiraz province (Iran). A total of 30 Salmonella isolates were found in chicken samples (15.6%), by conventional culturing and confirmed by PCR and serology methods. Strains of serogroup D1 were the most prevalent strains, followed by serogroups C1, B and C2. All strains were subjected to Salmonell...
متن کاملselective amplification of prt, tyv and inva genes by multiplex pcr for rapid detection of salmonella typhi
a multiplex pcr-based assay was developed for detection of salmonella typhi and identification of other salmonella serotypes. three primer-sets were selected from different genomic sequences, malo2-f/malo2-ra primers from invasion gene, parat-s/parat-as as well as tyv-s/tyv-as primers from o-antigen gene cluster of the genus salmonella. this method differentiated salmonella spp., based on size ...
متن کاملStudies on PCR-Based Rapid Detection Systems for Salmonella spp
1.1 Using molecular biological methods to identified Salmonella spp. As the industrialization had made food supply to exceed demand, more and more consumers were interested in ‘Delicious food’ than ‘Good quality food’ today and they ask for not only taste but also safety. Microbiological safety is one of the most critical factors for ensuring safe food supply. Fig 1. is a flow chart of traditio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Jundishapur Journal of Microbiology
سال: 2019
ISSN: 2008-3645,2008-4161
DOI: 10.5812/jjm.68764